Assessment of the quantity and purity of DNA obtained from buccal cells under different storage methods

Aim: This study aimed to verify the quantity and purity of DNA obtained from buccal cells under differentstorage conditions. Methods: Thirty students, between 18 23 years age participated in study. Three samples genetic material were collected each student (samples A, B, C) through a mouth rinse with 5 mL 3% glucose. The first phase extraction sample A was carried out on same day collection, whereas B C stored refrigerator freezer, respectively, for 1 month before phase. performed 10 M ammonium acetate mM EDTA. Sample assessed by spectrophotometry. Statistical analyses descriptive analysis variance ANOVA using SPSS software, version 21.0. Results: presented no statistically significant differences (p = 0.37) or quality 0.16). Conclusion: three satisfactory, storage conditions found. Uniterms: DNA. Mouth. Mucosa.